| 质粒名称: | pLenti CMV-IFP2.0 T2A HO1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 59427 |
| 存储实验室: | Xiaokun Shu |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti CMV/TO Puro DEST (670-1 |
| 载体出品公司: | addgene |
| 空载体大小: | 4038 bp |
| 完整质粒大小: | 11162 bp |
| 载体类型: | Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl2 |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | IFP2.0T2AHO1 IRES EGFP |
| 插入大小: | 3203bp |
| 启动子: | CMV |
| 克隆方法: | Gateway Cloning |
| 5’测序引物 | CMV-F |
|
文献引用方法 |
|
| 材料和方法部分: |
pLenti CMV-IFP2.0 T2A HO1 was a gift from Xiaokun Shu (Addgene plasmid # 59427 ; http://n2t.net/addgene:59427 ; RRID:Addgene_59427)
|
| 参考文献部分: | An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Yu D, Gustafson WC, Han C, Lafaye C, Noirclerc-Savoye M, Ge WP, Thayer DA, Huang H, Kornberg TB, Royant A, Jan LY, Jan YN, Weiss WA, Shu X. Nat Commun. 2014 May 15;5:3626. doi: 10.1038/ncomms4626. 10.1038/ncomms4626 PubMed 24832154 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLenti CMV-IFP2.0 T2A HO1 11162 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Express infrared fluorescent protein IFP2.0 plus HO1 enzyme.
ACCESSION .
VERSION .
KEYWORDS pLenti CMV-IFP2.0 T2A HO1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11162)
AUTHORS Yu D, Gustafson WC, Han C, Lafaye C, Noirclerc-Savoye M, Ge WP,
Thayer DA, Huang H, Kornberg TB, Royant A, Jan LY, Jan YN, Weiss WA,
Shu X
TITLE An improved monomeric infrared fluorescent protein for neuronal and
tumour brain imaging.
JOURNAL Nat Commun. 2014 May 15;5:3626. doi: 10.1038/ncomms4626.
PUBMED 24832154
REFERENCE 2 (bases 1 to 11162)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11162
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 27..131
/gene="bla"
/label=AmpR promoter
CDS 132..992
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(350..369)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1163..1751
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1652..1671
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1905..1922
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2039..2060
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2075..2105
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2113..2129
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2118..2140
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2137..2153
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2137..2153
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2172..2192
/label=T3
/note="T3 promoter, forward primer"
promoter 2174..2192
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
LTR 2448..2628
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2672..2797
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3290..3523
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 3707..3751
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 4012..4129
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 4152..4455
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4456..4659
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4610..4630
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 4661..4679
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 4682..4700
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 4741..4765
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 4786..5748
/codon_start=1
/product="bacteriophytochrome-based monomeric infrared
fluorescent protein (Yu et al., 2014)"
/label=IFP2.0
/translation="MARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLD
WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
VRELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLR
LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
QLWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
CDS 5752..5763
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 5755..5808
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 7269..7988
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(7314..7335)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(7575..7594)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 7922..7943
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind complement(7989..8013)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 8044..8632
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8097..8117)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8515..8526)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 8646..9145
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(8681..8704)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 9078..9097
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 9166..9765
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(9166..9185)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 9662..9682
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 9903..10136
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 10208..10329
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10245..10264)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10299..10318
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 10348..10483
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 10410..10429
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(10515..10534)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(10516..10534)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(10544..10561)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(10544..10560)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10553..10575)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 10705..11160
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(10792..10811)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 11002..11023
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct
4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
4321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
4381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
4441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
4501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
4561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
4621 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat
4681 ctccctatca gtgatagaga tcgtcgacta gtccagtgtg gtggaattct gcagatatca
4741 acaagtttgt acaaaaaagc aggctccgcg gccgccccct tcaccatggc tcgggaccct
4801 caacctttct tccctcctct gtatctcgga ggaccagaga tcactactga gaactgcgag
4861 cgggaaccta tacatatacc aggttctatt caacctcatg gagcactgct cacagccgat
4921 ggacattctg gcgaggtgct gcaagtttca ctcaacgcag ccactttcct gggccaggaa
4981 ccaactgttc tgcggggaca gaccctggca gccctgctgc cagaccagtg gcctgctctg
5041 cagactgccc tgcctccagg ctgccaggac gccctgcagt atcgcgccac cctggattgg
5101 cccgccgccg gacacctgag cctgaccgtg catagggtgg ccgagctgct gattctggaa
5161 ttcgagccta ctgaggcatg ggactctatt ggaccccacg ctctgagaaa tgcaatgttc
5221 gctctggaga gtgctcctaa tctccgggca ctggctgaag tcgcaaccca aacagtccgg
5281 gaactgtcag gtttcgaccg ggtgatgctg tacaagtttg caccagacgc aacaggagag
5341 gttattgccg aagcaaggcg cgagggcatg caggcttacc tcgggcatag gtttcccgca
5401 tccaccaccc ctgcacaagc tagggccctc tacacaagac acctgctccg gctgaccgca
5461 gacaccaggg ctgcagcagt gcccctcgac cccgtgctga atccccagac aaatgctcct
5521 acacctctgg gcggagctgt cctcagagct acatccccaa tgcacatgca gtacctgagg
5581 aatatgggag tgggctcctc cctgagcgtc agcgtcgtgg tcggcggcca gctgtgggga
5641 ctgattgtct gccaccatca gacaccctac gtgctgccac cagatctgcg gaccaccctg
5701 gagtatctgg ggaggctcct gtccctgcag gtgcagagga aagaagccga tatcgagggc
5761 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaat ggaaagacca
5821 cagccagact ctatgccaca ggatctgagc gaagccctca aggaggccac caaggaagtg
5881 cacacccagg ccgagaacgc tgagttcatg agaaacttcc agaaaggaca ggttactcgc
5941 gacggattca agctggtgat ggctagcctc tatcacatct acgtggccct ggaggaggaa
6001 attgagagaa acaaggaatc ccccgtgttc gctccagttt atttcccaga ggagctgcat
6061 cggaaggccg ccctggaaca ggatctggct ttttggtatg gcccccgctg gcaggaggtc
6121 attccttata cccccgctat gcaacgctac gttaagagac tccacgaggt gggccgcaca
6181 gaacctgagc tgctcgtggc ccatgcctac acccgctacc tgggcgacct gtcagggggt
6241 caagttctga aaaagatagc ccaaaaggcc ctggacctgc cttcctctgg cgaggggctg
6301 gccttcttta ctttccctaa cattgcctca gccacaaagt tcaagcagct ctaccggtcc
6361 aggatgaaca gcctggaaat gaccccagcc gtgcggcagc gggtcataga agaagccaag
6421 actgcctttc tgctgaatat ccagctgttt gaggaactcc aggaactgct gacccatgac
6481 actaaagacc agtccccctc cagggcccca ggactcagac agagggcctc aaacaaggtt
6541 caggactcag cccccgtgga aacacccagg ggaaaacccc ctctcaatac aagatctcaa
6601 gcacccctgc tgagatgggt cctcaccctg agcttcctcg tcgctaccgt tgcagtgggg
6661 ctctatgcta tgtgactcga gtactccggt attgcggtac ccttgtacgc ctgttttata
6721 ctcccttccc gtaacttaga cgcacaaaac caagttcaat agaagggggt acaaaccagt
6781 accaccacga acaagcactt ctgtttcccc ggtgatgtcg tatagactgc ttgcgtggtt
6841 gaaagcgacg gatccgttat ccgcttatgt acttcgagaa gcccagtacc acctcggaat
6901 cttcgatgcg ttgcgctcag cactcaaccc cagagtgtag cttaggctga tgagtctgga
6961 catccctcac cggtgacggt ggtccaggct gcgttggcgg cctacctatg gctaacgcca
7021 tgggacgcta gttgtgaaca aggtgtgaag agcctattga gctacataag aatcctccgg
7081 cccctgaatg cggctaatcc caacctcgga gcaggtggtc acaaaccagt gattggcctg
7141 tcgtaacgcg caagtccgtg gcggaaccga ctactttggg tgtccgtgtt tccttttatt
7201 ttattgtggc tgcttatggt gacaatcaca gattgttatc ataaagcgaa ttggatagga
7261 tccgtacgat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
7321 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
7381 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
7441 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
7501 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
7561 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
7621 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
7681 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
7741 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
7801 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
7861 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
7921 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
7981 acaagtaaac ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc
8041 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
8101 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
8161 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
8221 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
8281 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
8341 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
8401 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
8461 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
8521 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
8581 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
8641 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
8701 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
8761 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
8821 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
8881 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
8941 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
9001 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
9061 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
9121 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag
9181 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
9241 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
9301 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
9361 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
9421 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
9481 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
9541 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
9601 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
9661 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
9721 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
9781 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc cggtaccttt
9841 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
9901 actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
9961 tctctggtta gaccagatct gagcctggga gctctctggc tgcctaggga acccactgct
10021 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
10081 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
10141 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
10201 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
10261 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
10321 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat
10381 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
10441 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcgtcgag
10501 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
10561 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
10621 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
10681 gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
10741 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
10801 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
10861 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
10921 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
10981 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
11041 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
11101 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
11161 cc
//
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